Recent posts
immunology
Stem Cells of the Eye
Stem Cells of the Eye Introduction Two prerequisites are essential for good vision. The first is a clear optical focusing system. The main components of this (the cornea, lens and intraocular fluids) are designed to bring vis…
immunology
Solute Transport
SOLUTE TRANSPORT Transport proteins in the cell membranes of the nephron mediate the reabsorption and secretion of solutes and water transport in the kidneys. Acquired defects in transport proteins are the cause of many ki…
Subculture of Adherent Cell Lines
Procedure 1. View cultures using an inverted microscope to assess the degree of confluency and confirm the absence of bacterial and fungal contaminants. 2. Remove spent medium. 3. Wash the cell monolayer with PBS withou…
pharmacology
Acyclovir (topical, oral and parentral }
Acyclovir (topical, oral and parentral) MOA 1) A guanosine analogue, activated by viral thymidine kinase into Acyclo GMP. 2) Then converted by human cellular kinase into Acyclo GTP (active form). 3) AcycloGTP inhibits viral …
pharmacology
Antifungal Amphotericin B , side effects , use , MOA
Amphotericin B mechanism of actions Tightly and selectively bind to ergosterol in the plasma membranes ➔ formation of artificial pores which disrupt membrane function. ✓ Eit…
antibiotic
Beta-lactam antibiotics: Mechanisms of action and resistance and adverse effects
INTRODUCTION Beta-lactam antibiotics are among the most commonly prescribed drugs, grouped together based upon a shared structural feature, the beta-lactam ring. Beta-lactam antibio…
Stem Cells
Overview of Stem Cells
INTRODUCTION Stem cells are those cells that have the capability of self-renewal and differentiation. First identified in the hematopoietic system, they are likely to be present in many other tissues. Stem cells have altered the car…